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KMID : 0380019990140060655
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Korean Journal of Biotechnology and Bioengineering
1999 Volume.14 No. 6 p.655 ~ p.660
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Fractional Extraction of DHA and EPA by Supercritical Fluid
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Hur Byung-Ki
Noh Duck-Woo
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Abstract
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Supercritical fractional extraction of DHA and EPA from the mixture of fatty acids composing the fish oil was performed. The compositions, extracted quantities, and cumulative extracted quantities of fatty acids in the extract and the raffinate were investigated according to the fractional steps. The temperature and pressure for the miximum concentration of DHA and EPA in the extract or the raffinate were 60$^{\circ}C$ and 101 bar respectively among the extraction conditions considered in this study. In this case, the weight percent of EPA in the extract was 50% and that of DHA in the raffinate was 40%. These values were two times higher than those in fish oil. THe same temperature and pressure were used to extract DHA and EPA from the mixture of fatty acids whose the initial weight percent of DHA was 34%. The weight percent of DHA in the raffinate after the fourth fractionation was 7-%. And the remaining weight of DHA in the raffinate was 80% of DHA initially loaded in the extraction vessel.
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KEYWORD
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supercritical fluid extraction, fractional extraction, DHA(docosahexaenoic acid), EPA(eicosapentaenoic acid), fatty acid
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